FastQCFastQC Report
Sun 21 May 2023
EGAF00007962128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485004
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15110.3115438223189912No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14400.2969047677957295No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG14060.2898945163338859No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14040.2894821486008363No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13770.2839151842046664No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13150.27113178448012804No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12610.2599978556877881No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC11640.23999802063488138No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11500.23711144650353397No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG10340.2131941179866558No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9050.18659639920495502No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8960.1847407444062317No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG8190.16886458668382115No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC7830.16144196748892792No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT7510.15484408376013392No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT7450.15360698056098507No Hit
CGGACCAGGATCCGGACCGTCGTCGCCCATCTGATCCGGTTCCG7280.15010185483006325No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7260.14968948709701363No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6640.13690608737247528No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6620.13649371963942566No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6410.1321638584424046No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6130.12639071017970988No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6030.12432887151446173No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5970.12309176831531286No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5450.11237020725602263No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5250.10824652992552639No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5120.10556613966070383No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5120.10556613966070383No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5020.10350430099545571No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4950.10206101392978202No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT4920.10144246233020758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)