FastQCFastQC Report
Wed 8 May 2024
EGAF00007962130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454959
Total Bases20 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14040.3085992364147099No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13560.29804883516976255No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13550.29782903514382614No Hit
GATACGCGGTACACGAGGTTCGCGCGGGGAACGTGGCACACGCG12830.28200343327640515No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12350.2714530320314578No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11920.26200163091619244No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11490.2525502298009271No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA11070.2433186287115982No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC10680.23474642770007847No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG9710.21342582518424735No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8330.18309342160502376No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8310.18265382155315094No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC7990.17562022072318606No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7250.15935501880389222No Hit
CGGACCAGGATCCGGACCGTCGTCGCCCATCTGATCCGGTTCCG7050.15495901828516415No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG6900.1516620178961181No Hit
TACGGTATCAGGGGATTCCGGCTGCACCTCGGTTTCGCCTTTGT6750.14836501750707207No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT6740.14814521748113565No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6420.14111161665117078No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6070.13341861574339664No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5810.12770381506905018No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5640.12396721462813133No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5330.11715341382410284No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC5250.11539501361661161No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5060.11121881312381994No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5010.11011981299413794No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA4970.10924061289039233No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC4750.10440501231979145No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG4750.10440501231979145No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4730.10396541226791865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)