FastQCFastQC Report
Wed 31 May 2023
EGAF00007962164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495210
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23900.48262353345045533No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19370.3911471900809757No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17670.3568183195008178No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15550.31400819854203266No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13450.2716019466488964No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13030.2631206962702692No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13010.26271682720462025No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12480.252014296964924No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG12470.25181236243209953No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12000.24232143938934997No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11920.2407059631267543No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG11350.22919569475576018No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10990.22192605157407966No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG10540.21283899759697908No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG9140.1845681630015549No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9020.1821449486076614No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8350.1686153349084227No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8210.16578825144888026No Hit
AGAGCTGGTGCCAGAGTTCAGTGCGTTGACCAGTTCGTCAGCGT8200.16558631691605583No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8080.16316310252216232No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8070.16296116798933785No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG7970.16094182266109328No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7290.14721027442903012No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7250.14640253629773228No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG6730.13590194059086044No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6230.12580521394963753No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6140.12398780315421741No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG5780.1167181599725369No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG5770.11651622543971245No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG5710.1153046182427657No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5610.11328527291452112No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5550.11207366571757438No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5530.11166979665192545No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5460.11025625492215424No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5420.1094485167908564No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5410.10924658225803195No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5380.10864077865955857No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC5150.10399628440459603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)