FastQCFastQC Report
Wed 8 May 2024
EGAF00007962167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457008
Total Bases14.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72001.575464762104821No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23710.5188092987431292No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23050.5043675384238351No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15380.33653677834961315No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13580.2971501592969926No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13520.29583727199523857No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12970.28380247172916007No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12850.2811766971256521No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12780.2796449952736057No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12690.2776756643209747No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12320.2695795259601582No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11580.25338724923852535No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11520.25207436193677135No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10910.23872667436893885No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10650.2330374960613381No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10210.22340965584847527No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9780.21400063018590484No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9650.21115604103210447No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9500.20787382277771943No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9380.20524804817421138No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7920.17330112383153032No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7650.16739313097363723No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7510.1643297272695445No Hit
ACTGATGAGATTAGCTCCCGTATCCCGCAGC7090.1551395161572664No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6770.14813745054791164No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6520.14266708679060322No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6200.13566502118124846No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6160.1347897629800791No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6000.13128873017540174No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5890.12888177012218605No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5780.12647481006897035No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5640.12341140636487764No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5580.12209851906312362No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5460.11947274445961559No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5410.11837867170815392No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5410.11837867170815392No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5340.11684696985610754No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5250.11487763890347653No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5200.11378356615201483No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5190.11356475160172251No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4950.10831320239470645No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4710.10306165318769037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)