FastQCFastQC Report
Wed 8 May 2024
EGAF00007962169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498155
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37770.7581977496963797No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26160.5251377583282312No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24590.493621463199205No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17030.3418614688199456No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16000.321185173289438No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15550.3121518402906726No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14730.2956911001595889No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14630.2936836928265299No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14560.2922785076933886No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG13880.2786281378285875No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13790.2768214712288344No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13630.27360961949594004No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12890.2587548052313035No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12300.2469111019662555No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11940.23968443556724311No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11840.23767702823418416No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10870.21820517710351195No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10650.21378888097078216No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10300.2067629553050757No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10150.20375184430548726No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9180.18427999317481505No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8720.17504591944274372No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8540.17143258624323757No Hit
ACTGATGAGATTAGCTCCCGTATCCCGCAGC8090.1623992532444721No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8000.160592586644719No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7250.14553703164677662No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7140.1433288835804117No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7090.14232517991388224No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6920.13891258744768195No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6650.13349258764842267No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6420.12887555078238702No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6340.12726962491593982No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6210.12465999538296313No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6190.12425851391635134No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6130.12305406951651594No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6030.12104666218345696No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5860.11763406971725668No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5800.11642962531742129No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5590.11221406991799741No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5540.11121036625146791No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5290.10619184791882046No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5230.10498740351898506No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5170.10378295911914967No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5070.10177555178609067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)