FastQCFastQC Report
Wed 31 May 2023
EGAF00007962171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465342
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74221.5949559678687935No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24810.5331562592673775No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23820.5118815838673492No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16300.35028000911157814No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14830.3186903395782027No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14270.30665617975596443No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13810.2967709770448401No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13470.2894645228670526No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12670.2722728659781408No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12660.27205797026702944No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12550.26969411744480404No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11780.2531471476892264No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11560.24841944204477567No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11490.24691517206699587No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11220.24111298786698815No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10850.23316184655586641No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10240.22005320817807117No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9880.21231696257806085No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9660.2075892569336101No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9640.20715946551138734No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8780.1886784343558071No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7980.17148677746689533No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7830.16826334180022434No Hit
ACTGATGAGATTAGCTCCCGTATCCCGCAGC7530.1618164704668824No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7120.1530057463113151No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6870.14763335353353016No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6540.14054179506685405No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6260.13452471515573494No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6100.13108638377795256No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5990.1287225309557272No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5970.1282927395335044No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5950.1278629481112816No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5820.12506930386683343No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5780.12420972102238784No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5720.12292034675571945No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5490.1179777454001573No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5410.11625857971126613No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5390.11582878828904333No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5340.11475430973348635No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5090.1093819169557014No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5060.10873722982236719No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4960.10658827271125323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)