FastQCFastQC Report
Wed 8 May 2024
EGAF00007962179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492524
Total Bases15.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72831.478709666940088No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA16020.32526333742112057No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12470.25318563156313195No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12390.25156134523393786No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG8260.1677075634892919No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7470.15166773598850006No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7250.14720094858321625No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7050.14314023276023097No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7030.14273416117793244No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6200.12588219051254357No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5870.11918200940461784No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4950.10050271661888557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)