FastQCFastQC Report
Wed 8 May 2024
EGAF00007962220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454485
Total Bases19.9 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG30840.6785702498432291No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25310.5568940669108992No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG24190.5322507893549843No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG23020.5065073654796087No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22470.49440575596554337No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20600.4532602836177212No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG19500.4290570645895904No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19210.4226762159367196No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18610.40947446010319366No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17190.3782303046305158No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16060.3533669978107088No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14930.32850369099090176No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14570.32058263749078625No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG14250.31354170104623913No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13910.30606070607390784No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13380.29439915508762665No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA13370.29417912582373457No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12990.2858180137958348No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG12670.2787770773512877No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12310.2708560238511722No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11710.25765426801764635No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG11320.24907312672585455No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG11060.24335236586466No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10750.23653145868400496No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10510.23125075635059464No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10390.22861040518388945No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10330.22729022960053688No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG10260.2257500247532922No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG9880.21738891272539249No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT9820.2160687371420399No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9720.2138684445031189No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9720.2138684445031189No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9290.2044071861557587No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9280.20418715689186662No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9110.20044665940570097No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9090.20000660087791675No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8980.19758627897510367No Hit
ACGAGTCTCGATCATAGATTCATCAGAGGTGTTGGAGCTCGCAC8690.19120543032223286No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8610.18944519621109607No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8190.18020396712762796No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7890.17360308921086504No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7870.17316303068308084No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7250.1595212163217708No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7150.1573209236828498No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6990.15380045546057625No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6810.1498399287105185No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6760.148739782391058No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6640.14609943122435284No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG6520.14345908005764768No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6210.13663817287699265No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT5980.1315774998074744No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5960.1311374412796902No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5850.1287171193768771No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5800.12761697305741665No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5790.12739694379352454No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5680.12497662189071147No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5630.12387647557125098No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5560.1223362707240063No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5500.12101609514065371No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5350.11771565618227224No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5290.11639548059891966No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4960.10913451489048044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)