FastQCFastQC Report
Wed 31 May 2023
EGAF00007962221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454485
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47841.0526199984597953No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG33770.7430388241636138No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA29740.6543670308150984No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28320.6231228753424205No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG27370.6022200952726713No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27270.6000198026337502No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG26500.5830775493140589No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24170.5318107308272No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG23290.5124481556046955No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC23010.5062873362157166No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG19450.4279569182701299No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG19350.425756625631209No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC17990.3958326457418837No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16370.3601879049913638No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG15920.35028658811621943No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14610.32146275454635465No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG14450.3179422863240811No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13830.30430047196277105No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT13500.29703950625433184No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13340.29351903803205825No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12690.27921713587907193No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC11900.2618348240315962No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11750.25853438507321475No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11570.254573858323157No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG11420.25127341936477554No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG11330.24929315598974663No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11230.24709286335082564No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10830.23829169279514176No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG10520.23147078561448672No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG10480.23059066855891835No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10140.223109673586587No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10030.22068935168377393No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9870.21716888346150037No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9350.20572736173911133No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9110.20044665940570097No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8850.19472589854450642No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8660.19054534253055655No Hit
GACTCCGTTTCTCGTGCGCTGACTCAGGCAC8300.18262428903044106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8190.18020396712762796No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8030.1766834989053544No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7870.17316303068308084No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7780.17118276730805196No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7760.1707427087802678No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7610.1674422698218863No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7360.16194153822458388No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7280.16018130411344708No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6460.14213890447429509No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6360.1399386118353741No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6230.13707823140477685No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6200.13641814361310053No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6150.13531799729364005No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5890.12959723643244553No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5670.12475659262681936No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5590.1229963585156826No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5510.1212361244045458No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5460.1201359780850853No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5400.11881580250173274No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5360.11793568544616435No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5190.11419518795999868No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4800.10561404666820688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)