FastQCFastQC Report
Wed 8 May 2024
EGAF00007962222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428605
Total Bases18.8 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG30590.713710759323853No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25370.5919202995765331No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21910.5111932898589611No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG21840.5095600844600506No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21830.5093267694030634No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20220.4717630452281238No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18220.4251000338306833No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG18170.42393345854574727No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18150.42346682843177286No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15740.36723789969785703No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG15240.35557214684849686No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14020.32710770989605814No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG13190.3077425601661203No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13160.3070426149951587No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13130.3063426698241971No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA13120.3061093547672099No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13100.3056427246532355No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13050.3044761493682995No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG12190.28441105446740006No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11860.27671165758682237No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11460.26737905530733425No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG10740.25058037120425564No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10390.24241434420970356No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG10380.2421810291527164No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10110.2358815226140619No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG9730.22701555044854818No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9690.2260822902205994No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9640.22491571493566337No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9400.2193161535679705No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9250.21581642771316248No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT9110.21255001691534164No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8860.20671714049066156No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8800.20531725014873836No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8620.20111757912296868No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8360.19505138764130142No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8260.1927182370714294No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG8250.1924849220144422No Hit
ACGAGTCTCGATCATAGATTCATCAGAGGTGTTGGAGCTCGCAC8100.18898519615963416No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7860.18338563479194128No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7620.17778607342424843No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7430.17335308734149157No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7230.16868678620174754No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6990.16308722483405466No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG6690.15608777312443858No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6610.15422125266854098No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6510.15188810209866893No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6440.1502548966997585No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6430.15002158164277132No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6280.14652185578796328No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6140.14325544499014245No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT5950.1388224589073856No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5710.13322289753969271No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5520.12878991145693586No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5500.12832328134296148No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5410.12622344583007664No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5350.12482355548815342No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5310.12389029526020462No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5270.1229570350322558No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5160.12039056940539657No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5120.11945730917744778No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5000.11665752849360134No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4400.10265862507436918No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4390.10242531001738196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)