FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433159
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG28820.6653445963260605No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25890.5977019985732722No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG22180.5120521563675232No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21940.5065114657666123No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG21440.4949683603480477No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19220.4437169722896211No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG18400.42478627940317526No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18190.41993817512737813No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18000.4155517950683236No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16020.369841097610808No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG15210.35114126683273345No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG14010.3234378138281786No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13770.3178971232272676No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13380.3088935010007872No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13240.30566143148358915No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12690.29296401552316814No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA12650.292040567089683No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12520.28903935968085626No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG11980.2765728058288065No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG11480.265029700410242No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11330.2615667687846726No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11330.2615667687846726No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG10660.2460990075237961No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10180.23501762632197415No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9980.23040038415454833No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9910.2287843493959493No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9820.22670659042060767No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC9560.22070417560295408No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9460.2183955545192412No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT9380.21654865765227088No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG9350.215856071327157No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9270.2140091744601867No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG8900.20546727645044893No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8790.20292779325836469No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8630.19923399952442405No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8570.1978488268741963No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8470.1955402057904834No Hit
ACGAGTCTCGATCATAGATTCATCAGAGGTGTTGGAGCTCGCAC8330.19230813627328533No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8180.18884520464771598No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7980.18422796248029016No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7890.18215020350494854No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7580.1749934781454385No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7380.17037623597801269No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7100.16391209694361655No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7040.1625269242933888No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6770.15629364736736395No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6510.15029123254971039No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6490.1498295083329678No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6280.1449814040571707No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6070.1401332997813736No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5930.13690123026417553No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5850.13505433339720518No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5740.132514850205121No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5650.13043709122977937No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5550.12812847014606646No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5520.12743588382095258No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5230.12074088267818514No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5210.12027915846144256No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5180.1195865721363287No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4920.11358415731867513No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4890.11289157099356126No Hit
CGGCTGTTCGTTGTCGACTTCGATAATTGCAACGCAAGCGCCGC4420.10204105190011058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)