FastQCFastQC Report
Sun 21 May 2023
EGAF00007962225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433159
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT43781.0107143104495115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37910.8751982528355639No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG31200.7202897781184276No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27970.6457213171145006No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27850.6429509718140451No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27150.6267906242280549No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG25250.5829268236375095No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24520.5660738897264053No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22090.5099743973921816No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC21960.5069731899833548No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21500.49635353299827545No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG18890.4360985227133686No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG18310.4227085204278337No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC17250.3982371369404768No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14860.3430610930397383No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG14850.342830230931367No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA13840.3195131579858666No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13630.31466505371006953No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG13160.30381453461661884No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12550.2897319460059701No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT12430.28696160070551463No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG11720.27057039101115293No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11290.2606433203511874No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC11220.2590272855925884No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11180.25810383715910323No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10980.2534865949916774No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10720.24748418017402385No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG10510.24263607589822675No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10480.24194348957311287No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9830.22693745252897898No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9780.22578314198712252No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9590.221396761928068No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9460.2183955545192412No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9400.21701038186901345No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9200.21239313970158763No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8550.1973871026574537No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8540.19715624054908246No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8460.1953093436821121No Hit
GACTCCGTTTCTCGTGCGCTGACTCAGGCAC7960.18376623826354757No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7780.17961072031286435No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7770.17937985820449304No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7510.17337744338683947No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7480.1726848570617256No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7380.17037623597801269No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7140.1648355453771017No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6850.15814054423433427No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6250.14428881773205682No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6220.14359623140694294No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5990.13828640291440325No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5820.1343617470720913No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5720.1320531259883784No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5680.13112967755489324No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5630.1299753670130368No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5610.1295136427962942No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5550.12812847014606646No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5270.1216643311116703No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5200.12004829635307128No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5000.11543105418564546No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4980.11496932996890288No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4590.10596570774242253No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT4560.10527312141730864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)