FastQCFastQC Report
Wed 8 May 2024
EGAF00007962363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457304
Total Bases14.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT38600.8440774626944002No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30820.6739499326487413No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG27100.592603607228452No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT26180.5724856987911762No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25670.5613333799835558No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA25410.5556478841208473No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24640.5388100694505187No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24380.5331245735878103No Hit
GGCGAACCGCCACGCGCACGTGGTGCAGTAC17300.3783041477879048No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15910.3479086122141945No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15650.3422231163514861No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15600.3411297517625037No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG15250.33347619963962705No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13860.30308066406591677No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT13670.2989258786277837No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13570.29673914944981894No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG10920.2387908262337526No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT10470.22895054493291114No Hit
TTCCGCAACCACCACCTGATCGGCAAACAGA9830.21495547819393662No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9820.21473680527614017No Hit
GAACTGAACCCATCCGGTAACTTCGAATCCG9050.19789899060581145No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9040.197680317688015No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8970.19614960726343963No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8950.1957122614278467No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG8880.19418155100327134No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8680.1898080926473418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8310.18171719468887218No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7770.16990885712786244No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7720.16881549253888004No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7690.16815947378549062No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7000.15307104245753372No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA6890.1506656403617725No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6840.1495722757727901No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6680.14607350908804648No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6670.14585483617024997No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6660.14563616325245352No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6500.14213739656770988No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6420.14038801322533806No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6260.13688924654059445No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6240.1364519007050015No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA5730.12529958189738116No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5630.12311285271941641No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG5470.11961408603467277No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5170.11305389850077847No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5170.11305389850077847No Hit
CCGCCACAGACCTCCGGCCAGGTTTACCTGC5040.11021115056942428No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG4810.1051816734601053No Hit
TCCGAAGCGGATCTGAACAAAGCGAGCCTGG4750.10386963595332645No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4730.10343229011773351No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT4620.10102688802197225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)