FastQCFastQC Report
Wed 8 May 2024
EGAF00007962366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461694
Total Bases20.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT36950.8003136276408184No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT24760.5362859383054577No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG24420.5289217533691146No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23360.505962823861692No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20420.4422842835297838No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG18800.4071961082448548No Hit
GAAAGTGCGCAGTTCACGCGGCGCGCTGGAGTTTTCAAACAGGC17340.37557343175349905No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT14540.31492720286596754No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC14010.30344773811225617No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13480.2919682733585448No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA13290.2878529935411766No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG13120.2841709010730051No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13060.2828713390254151No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12910.2796224339064402No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12710.27529056041447364No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG12250.2653272513829506No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA12040.2607787842163858No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10580.22915610772503No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA10300.22309148483627686No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT10140.21962598604270359No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA10140.21962598604270359No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9010.19515090081309266No Hit
CTGCTCTGGGGTGCGAACAGAATGGGTAGCCACAACCTTACCGT8710.18865309057514285No Hit
AAATTCACGAACGATGCGTGGGCCCGGCGGGGTCGGTGCCACCG8480.18367143605938133No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8080.17500768907544825No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8050.17435790805165324No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7940.17197537763107165No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7920.171542190281875No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT7880.1706758155834817No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7700.16677712944071182No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC7480.16201206859954861No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6990.1513989785442306No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6960.1507491975204356No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6720.14555094933007576No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6490.14056929481431424No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6450.13970292011592095No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6320.13688720234614268No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5930.12844004903680792No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5920.1282234553622096No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT5590.12107586410046482No Hit
AACGCTCTGGGTGTGTTTCGGACCAGAGGAAACGCTGGCCAGGG5330.11544442856090832No Hit
GTCTGCATTTAAATAGTTAACTCAATCAGACGGACGGGTCGGCA5320.11522783488630997No Hit
CGGACCGCTAGATACAGACGCCAGGGTTTGTTTGGTTTTTTCCA5150.11154574241813843No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA5050.10937980567215515No Hit
CGGTTTATTGCCATCTTCCTTACCCGGTTTGTTGCCATCCTCTT4950.10721386892617187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)