FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461694
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37740.8174245279340862No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30260.6554124593345376No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG27470.5949828241216043No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27180.588701607558253No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA25820.5592448678128804No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT25700.5566457437177005No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG25360.5492815587813573No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24570.5321706584880895No Hit
GGCGAACCGCCACGCGCACGTGGTGCAGTAC17080.3699419962139426No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG16020.3469830667065199No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15770.34156822484156174No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15390.3333376652068253No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14940.3235909498499006No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13900.3010652076916746No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13380.28980233661256155No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT13360.2893691492633649No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT10900.23608710531217644No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG10250.2220085164632852No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9950.21551070622533539No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9400.2035980541224274No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9310.20164871105104246No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9140.1979666185828709No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9040.19580068183688765No Hit
TTCCGCAACCACCACCTGATCGGCAAACAGA8990.194717713463896No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG8940.19363474509090436No Hit
GAACTGAACCCATCCGGTAACTTCGAATCCG8830.19125221467032277No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8760.1897360589481345No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7980.17284175232946497No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7930.17175878395647332No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7620.1650443800439252No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7470.1617954749249503No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7260.15724700775838543No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG7100.15378150896481219No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6970.15096579119503395No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6850.14836666709985402No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6670.14446798095708413No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6670.14446798095708413No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC6390.13840335806833098No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6140.13298851620337282No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5990.12973961108439788No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5900.12779026801301296No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5870.12714048698921795No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG5790.12540773759243135No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA5670.12280861349725143No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5370.11631080325930161No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5180.1121955234419334No Hit
CCGCCACAGACCTCCGGCCAGGTTTACCTGC5170.11197892976733508No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT4960.10743046260077022No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4900.10613090055318025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)