FastQCFastQC Report
Wed 8 May 2024
EGAF00007962401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433919
Total Bases13.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA30210.6962128876587567No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21650.4989410466008633No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19150.44132660703956267No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19040.4387915716988654No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18360.42312044413819166No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG18270.42104632431398487No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15790.36389280026917464No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15020.34614755288429405No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13720.31618804431241776No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12770.2942945572791235No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11710.26986603490513206No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11200.2581126892346267No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11110.2560385694104199No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10460.24105881512448174No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9570.22054807464065873No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9100.2097165600031342No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8970.20672060914594662No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA8770.20211145398104255No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8400.19358451692597006No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8200.188975361761066No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8090.18644032642036878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7890.18183117125546472No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7550.17399560747512785No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7080.16316409283760333No Hit
ACTAGCTGTCCGTTCACCCTGCCTCGTGGTG7050.1624727195628677No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7050.1624727195628677No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG6980.1608595152551513No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC6880.1585549376726993No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6600.15210212044183363No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6320.14564930321096795No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC6150.1417315213207995No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6140.14150106356255432No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG5980.13781373943063108No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC5940.13689190839765025No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5920.13643099288115987No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5600.12905634461731336No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5480.12629085151837094No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5440.12536902048539011No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5050.11638116791382724No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5020.11568979463909163No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4890.112693843781904No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA4860.11200247050716838No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4860.11200247050716838No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4710.10854560413349035No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4690.10808468861699995No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4630.10670194206752874No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4460.1027841601773603No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4450.10255370241911507No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4430.10209278690262469No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG4430.10209278690262469No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4350.10024912483666305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)