FastQCFastQC Report
Wed 8 May 2024
EGAF00007962405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438965
Total Bases13.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA30690.6991445787249553No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22520.5130249564316062No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20550.46814666317360154No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG18690.42577426446299815No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18230.41529506908295655No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18060.41142232296424547No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT16430.37428952194366294No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA15220.3467246819222489No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13250.3018463886642443No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA12690.28908910733201965No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11660.26562482202453497No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11460.26106865012016905No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11420.26015741573929585No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10770.24534985705010648No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA9790.22302461471871335No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9630.21937967719522058No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9290.21163418495779845No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9000.20502773569646784No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8970.20434430991081295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8380.1909036027929334No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8360.1904479856024968No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8340.1899923684120602No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7420.1690339776519768No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7350.1674393174854487No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7350.1674393174854487No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA7330.1669837002950121No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7220.16447780574761087No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC7100.16174410260499128No Hit
ACTAGCTGTCCGTTCACCCTGCCTCGTGGTG6700.1526317587962594No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6690.15240395020104108No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6660.1517205244153862No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC6130.13964666886881644No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6060.13805200870228834No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6040.13759639151185174No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6020.13714077432141514No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5840.13304021960748577No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5760.13121775084573942No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5160.11754923513264157No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5030.11458772339480369No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5030.11458772339480369No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4980.1134486804187122No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4920.11208182884740242No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG4900.11162621165696582No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4850.11048716868087433No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4850.11048716868087433No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4680.10661442256216327No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4620.1052475709908535No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4600.10479195380041688No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG4570.10410852801476199No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4450.10137482487214242No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA4440.10114701627692413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)