FastQCFastQC Report
Wed 8 May 2024
EGAF00007962429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515627
Total Bases15.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA67411.307340383649421No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA37700.7311486791808808No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32330.627003628591986No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17280.3351259728447114No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14040.27228985293632796No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12060.23389000188120485No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11700.22690821078027332No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10940.21216887401164017No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG10390.2015022487185504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9960.19316288712577115No Hit
ATCGATGATGTTAAAGGTCGTGTGCCGATGA8200.15902968618788388No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8040.15592666792080323No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC7490.14526004262771344No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6820.13226615363431318No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC6430.12470254660830406No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6190.12004801920768307No Hit
GCGGGCGATTCCGAGATTGGCGGTAAAGGCG5350.1037571733055096No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG5260.10201172553027672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)