FastQCFastQC Report
Wed 8 May 2024
EGAF00007962440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443637
Total Bases19.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30530.6881752423715786No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28890.6512080822834885No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21400.48237635724702854No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19380.43684363567511275No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16980.38274535261937126No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT15830.3568232586551618No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12720.28672090019543006No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12400.27950779578799784No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12140.2736471484569592No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12020.2709422343041721No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10700.24118817862351427No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10460.23577835031794014No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC9220.20782757073914032No Hit
GTAATCGTTGCGTGGCCAGATGTACGGAGAGTTGTGGTCGTATG9050.20399560902269195No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8880.20016364730624361No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8590.19362677143700818No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8420.18979480972055982No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC8380.1888931716696308No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8130.18325793385132438No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7480.16860631552372773No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7050.1589137064762407No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG6990.15756124939984717No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6770.15260224011973753No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6700.15102437353061174No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6580.14831945937782465No Hit
ACGCAGGGTGTCTTTGAAGAAGCCCATGTAATATTCACGGTAAG6570.1480940498650924No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6490.14629077376323435No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6470.14583995473776984No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6460.14561454522503758No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5910.1332170220247635No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5710.12870883177011835No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5610.1264547366427958No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5350.12059408931175714No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC5260.11856540369716684No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5010.11293016587886043No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5000.11270475636612817No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4940.11135229928973461No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4900.11045066123880559No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4860.10954902318787657No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4640.10459001390776693No Hit
CTGTTTTTTACGCGCGCCACGTGGTGCGGTATCGTGCGGCTGAC4580.10323755683137341No Hit
CTGTTTTTTACGAGCACCGCGCGGTGCGGTATCGTGTGGCTGAC4460.10053264267858632No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4440.10008182365312181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)