FastQCFastQC Report
Wed 31 May 2023
EGAF00007962441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443637
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT73251.6511246807637776No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31810.7170276600013074No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20290.4573559013337481No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19270.4343641310350579No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16430.37034782941909716No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14240.32098314613073303No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13330.3004708804720977No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13040.2939340046028623No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12990.292806957039201No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12920.29122909045007517No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12400.27950779578799784No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10940.24659800692908843No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10780.2429914547253723No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG9920.22360623663039828No Hit
TACGACGACTTCCATACCGATGAAGATAAAC9560.21549149417203706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.20196692340810168No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8780.19790955217892106No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8660.19520463802613397No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8570.19317595241154367No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7530.169733363087389No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7470.16838090601099548No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7450.16793008698553097No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7120.16049157306536652No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6880.15508174475979236No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6760.15237683060700527No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6680.15057355450514723No Hit
GTTCTGCAGGGTGTTCAGAGCTTTGTGGGCG6490.14629077376323435No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6200.13975389789399892No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5830.13141374592290544No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5800.13073751738470865No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5460.12307359395181194No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5430.12239736541361519No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5400.12172113687541843No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5350.12059408931175714No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5280.11901622272263133No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5270.1187908132098991No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5000.11270475636612817No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4860.10954902318787657No Hit
CCGAGCACTAAACCGCGTCCGAAAAACCCTC4850.10932361367514432No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4800.10819656611148305No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4720.10639329000962498No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4650.1048154234204992No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT4570.10301214731864114No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4530.10211050926771212No Hit
CTGGCCTCTTCTGCACCGTCCACCGCGGTTG4450.10030723316585408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)