FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447145
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70481.5762224781670375No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31920.7138623936307015No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21000.46964631159914566No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19520.436547428686444No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16750.37459884377550906No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14310.32003041518970354No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13410.29990271612116876No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13340.29833722841583826No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13280.2969953818112693No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12860.2876024555792864No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12650.2829059924632949No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10950.24488700533384025No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9970.22297017745921346No Hit
TACGACGACTTCCATACCGATGAAGATAAAC9870.2207337664515985No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG9640.21559002113408404No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8920.19948786187925618No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8740.1954623220655492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8480.18964765344575027No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8240.18428026702747433No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7790.1742164174932069No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7720.1726509297878764No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7480.16728354336960047No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7310.16348164465665502No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7100.15878518154066354No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7000.15654877053304855No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6950.15543056502924107No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6540.14626127989801965No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6470.14469579219268916No Hit
GTTCTGCAGGGTGTTCAGAGCTTTGTGGGCG6450.14424850999116617No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5980.13373737825537577No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5420.12121347661273188No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5340.1194243478066399No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5240.11718793679902492No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5190.11606973129521744No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5060.11316239698531795No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5050.11293875588455646No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5040.11271511478379496No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4920.11003142157465699No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT4850.1084659338693265No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4740.10600588176095004No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4720.10555859955942704No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4700.10511131735790402No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4540.10153305974572008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)