FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532871
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG44570.8364125651424078No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT21490.40328709950438285No Hit
CATGCTGTCCTGCAGCGGCATCGGTGGTGGCGGGAAGCGGGTAG19810.37175976924996856No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT19270.3616259845253354No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT18440.3460499820782141No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16900.31714992934500097No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC15120.28374597228972864No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14810.2779284292070689No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12020.22557054146313088No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11950.22425690270253024No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT11580.21731338353935567No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG11550.21675039549909828No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11450.21487376869824026No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG11080.2079302495350657No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11020.2068042734545509No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10950.20549063469395032No Hit
AACCAGCGGTACCAGGGACAGCTGCAGGCGTGGCGGACCGGTTT10390.19498152460914556No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10350.19423087388880234No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA9410.1765905819607372No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9330.17508928052005082No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8950.16795809867679043No Hit
CCACTGTTCCGGAACCAGTGGACCTTCCATCGGCTGCTGAGGCA8850.16608147187593245No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG8470.15895029003267208No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG8180.1535080723101839No Hit
CATGGAGTCCTGCAGCGGCATCGGCGGAGGCGGGAAGCGGGTTG8150.15294508426992648No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8060.1512561201491543No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7860.1475028665474383No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7820.14675221582709513No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7580.14224831150503592No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7370.13830739522323415No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7310.13718141914271934No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6860.12873659853885838No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6800.12761062245834356No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA6650.12479568225705659No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6620.12423269421679918No Hit
CACCAGCGGAACCAGGGACAGCTGCAGACGCGGAGGACCGGTTT6290.11803982577396782No Hit
CTGAGGCTGGGTCGCCGGAACCGGTGGAACCGGACCCATGCTGG6040.11334825877182281No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5900.11072098125062162No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT5780.10846902908959204No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA5640.10584175156839085No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5510.10340213672727545No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5480.10283914868701804No Hit
CATAGAGTCCTGCAGCGGCATCGGCGGCGGCGGGAAACGGGTCG5340.10021187116581685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)