FastQCFastQC Report
Wed 8 May 2024
EGAF00007962457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532871
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG42870.804509909527822No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23040.4323748149176817No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21920.4113565947480722No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA21820.40947996794721425No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT18980.35618376680284725No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC18760.3520551878409596No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18100.3396694509552969No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15190.2850596110503293No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC15060.28261999620921385No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13710.25728553439763097No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12980.24358615875136758No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12500.23457835010724923No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11810.22162962518132906No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11750.22050364910081427No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11700.21956533570038528No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11610.2178763715796131No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11220.21055752705626693No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10770.20211270645240595No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC10490.19685815141000357No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9480.17790422072133782No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9400.1764029192806514No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9230.1732126537191928No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG9090.17058537619799163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9040.16964706279756264No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8550.16045159147335847No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG8300.15576002447121348No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCAC8250.1548217110707845No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8110.1521944335495833No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8080.1516314455093259No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7680.14412493830589393No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7460.13999635934400634No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA7040.13211452678040275No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6900.12948724925920158No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6740.12648464637782877No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6710.12592165833757138No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC6280.11785216309388201No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC6190.11616319897310981No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6160.11560021093285243No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5930.11128396929087903No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA5850.10978266785019264No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG5830.10940734249002104No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5690.10678006496881985No Hit
GGTCACGGTCAGGACCAGGTTAGCCAGTTCC5420.10171317260650327No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5410.10152550992641746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)