FastQCFastQC Report
Wed 8 May 2024
EGAF00007962461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531218
Total Bases16.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG42090.7923300791765339No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22750.42826109055039546No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA21360.40209480853434937No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20580.38741157114405006No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT19100.3595510694291233No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC18970.3571038631974067No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17620.3316905677141964No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC15300.2880173488097165No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14280.2688161922224021No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12800.24095569050747528No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12750.24001445734143045No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12720.23944971744180354No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12160.2289079059821015No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11960.22514297331792224No Hit
GTTCACGGCCACACCCAGGGTCCGTGGGACC11930.2245782334182953No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11930.2245782334182953No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11660.2194955743216533No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11560.2176131079895636No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10620.1999179244679209No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC10000.18824663320896506No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9560.1799637813477706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9330.1756341087839644No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9300.17506936888433752No Hit
GTGGCCCCGCTGCGTGCGTCCATGGGTCCGG9170.17262216265262095No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCAC8820.16603353049030717No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8430.15869191179515754No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8350.15718593872948583No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8330.15680944546306788No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7960.14984432003433618No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG7680.14457341430448517No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA7450.14024374174067897No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG7340.13817302877538035No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7330.13798478214217139No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA7000.13177264324627555No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6950.13083141008023072No Hit
TTCTCCCAGGTTGCTGACGTAGTCCGTGCAC6320.11897187218806592No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6310.11878362555485696No Hit
GTACACGGCCACACCCAGGGCCCGTGGGACC6180.1163364193231404No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5970.11238324002575213No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5770.10861830736157284No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5750.10824181409515492No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG5710.10748882756231905No Hit
CCGCACCTGCAGAGCGAAACCGGTCCGCCGC5610.1056063612302294No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5590.10522986796381145No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5470.10297090836530388No Hit
GGTCACGGTCAGGACCAGGTTAGCCAGTTCC5450.10259441509888596No Hit

[OK]Adapter Content

Adapter graph