FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007962504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549982
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT28690.5216534359306304No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26510.48201577506172927No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT23180.42146833896382063No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16890.30710095966777096No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT16170.294009622133088No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15920.28946401882243417No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10850.19727918368237507No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10780.19600641475539202No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10550.1918244597095905No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10290.18709703226651053No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10060.18291507722070904No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10030.1823696048234306No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC9820.1785512980424814No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9560.17382387059940144No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC9490.17255110167241838No Hit
GTCCTGATCTTTCTTCGGTGGGATTGCCATGAAGCTCAGGTGCG9190.1670963776996338No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8940.16255077438898No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8870.16127800546199694No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT8480.154186864297377No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8270.1503685575164278No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7980.14509565767606938No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7800.14182282329239868No Hit
CTCCAGGGTCGCGACGGTGTTTTCGATTGCTTCCGTAGTCGGGG7660.1392772854384325No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7290.1325497925386649No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA7260.13200432014138644No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7110.12927695815499418No Hit
GGTCGGGGTGCTGGTTGGTTCACCAGAGGCGATGGTGTTGATAG6960.12654959616860187No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6950.12636777203617575No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6830.12418588244706191No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6810.1238222341822096No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6790.12345858591735728No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6640.12073122393096501No Hit
GGTGGAGTGCACAGTCGGTTCTGCGCTGGCGATGGTGTTAATCG6420.11673109301758967No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA6320.11491285169332814No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6240.11345825863391892No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5750.10454887614503748No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5650.10273063482077595No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5500.10000327283438368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)