FastQCFastQC Report
Wed 8 May 2024
EGAF00007962505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549982
Total Bases17 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44140.8025717205290356No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35310.6420210115967432No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28660.521107963533352No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22760.4138317254019222No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17740.3225560109239939No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17660.3211014178645847No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16290.2961915117222018No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT16090.2925550290736788No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12530.2278256379299686No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12480.22691651726783785No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11110.20200661112545504No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10650.193642701033852No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10440.18982439425290284No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10160.18473331854497055No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG9920.1803695393667429No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC9800.17818764977762908No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9530.17327839820212299No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9350.17000556381845225No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9200.16727820183205996No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8760.15927794000530926No Hit
GCCAAACCGGTAGCGGTTCGCTCTCCGGCAC8410.15291409537039394No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8390.15255044710554164No Hit
ACCATGACCCGTCACCCGCACCCACATCTGT8300.15091402991370628No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8050.14636842660305246No Hit
ACCGTCCCGGCAAAATCCTGCCAGGCTCAGC7660.1392772854384325No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7530.13691357171689256No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7370.13400438559807412No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7250.1318224960089603No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7150.13000425468469878No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG7080.1287314857577157No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7080.1287314857577157No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6880.12509500310919267No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6700.12182216872552193No Hit
ACCGTTCCTGCCAAATCCTGCCAGGACCAGC6320.11491285169332814No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6280.11418555516362354No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6050.11000360011782205No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5700.10363975548290671No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5610.10200333829107133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)