FastQCFastQC Report
Wed 8 May 2024
EGAF00007962515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557366
Total Bases17.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70691.2682869066286784No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34710.6227505804085646No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25960.4657621742266303No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24680.44279701309373015No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG23980.4302379405991754No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23230.41678179149786676No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21020.3771310054793439No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19710.35362759838239144No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18010.32312699375275855No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17540.3146944736492718No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16600.29782943344229823No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15880.2849115303050419No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15470.27755550212965985No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15010.26930239734752387No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14950.2682259054194192No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13300.23862237739654016No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11900.21350423240743066No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA11770.2111718332298705No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11560.20740411148150406No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11450.20543054294664548No Hit
TCCTACACCAACGGCAAACTGACTATCACCC11350.20363638973313766No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10870.19502445430830012No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10620.19053907127453057No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10600.190180240631829No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10590.1900008253104782No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10220.18336245842049928No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9980.17905649070808052No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA9800.17582701492376643No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG9560.17152104721134764No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9350.16775332546298125No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9230.16560034160677187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9150.1641650190359656No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8890.15950022068084527No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8540.1532206844335679No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8410.15088828525600773No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8190.1469411481862905No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC8130.14586465625818584No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8090.1451469949727827No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7910.14191751918846862No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7460.1338438297276834No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7340.13169084587147403No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG7240.12989669265796622No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7180.12882020072986153No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7090.1272054628377045No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7020.12594955558824902No Hit
TGCCAGCACCCGGAACCGCTGGCTTGCCCAG7020.12594955558824902No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA6570.11787586612746381No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6410.11500522098585131No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6110.10962276134532783No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG6020.1080080234531708No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5930.10639328556101377No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5810.10424030170480439No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5780.10370205574075203No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5750.10316380977669969No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5750.10316380977669969No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5740.10298439445534892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)