FastQCFastQC Report
Wed 8 May 2024
EGAF00007962517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533477
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT65371.2253574193451637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44040.8255276234964206No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32850.6157716265181067No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23670.4436929802034577No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23340.4375071465124082No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG23290.4365698989834613No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21270.39870509881400695No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19350.3627147937024464No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17450.3270993876024646No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16970.31810181132457443No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16470.30872933603510555No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16270.304980345919318No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14800.2774252685682794No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14620.27405117746407065No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT14040.2631791061282867No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13770.25811796947197346No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12230.22925074558040928No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA11470.21500458314041654No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11410.21387988610568032No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11170.2093810979667352No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10870.20375761279305388No Hit
TCCTACACCAACGGCAAACTGACTATCACCC10640.19944627415989816No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10300.19307299096305933No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10280.19269809195148058No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9960.18669970776622047No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA9830.18426286419095855No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9760.18295071765043291No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9050.16964180273938706No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8860.1660802621293889No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8800.16495556509465265No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8760.16420576707149512No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG8640.1619563730020226No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8250.15464584227623684No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8090.1516466501836068No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC7980.14958470561992362No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7790.14602316500992546No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7720.14471101846939982No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7650.14339887192887416No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7630.1430239729172954No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7430.13927498280150782No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7330.13740048774361407No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG7280.1364632402146672No Hit
TGCCAGCACCCGGAACCGCTGGCTTGCCCAG6950.1302774065236177No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA6950.1302774065236177No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6930.12990250751203894No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6710.12577861838467264No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6020.11284460248520554No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5930.11115755693310114No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5790.10853326385204985No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5670.10628386978257733No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5660.10609642027678795No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5550.10403447571310478No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG5490.10290977867836852No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5370.10066038460889598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)