FastQCFastQC Report
Sun 21 May 2023
EGAF00007962519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554919
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68091.2270259263063619No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34590.6233342163450882No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25880.46637437175515706No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25060.4515974403471497No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24930.44925475609953885No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG22470.404923961875517No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21130.38077629347706604No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19600.35320470194749143No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19100.3441943779182187No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17090.3079728753205423No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16750.3018458549806368No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16050.28923140133965497No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15440.27823880602394224No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15280.27535550233457495No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14750.26580455886354587No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13020.23462883772226217No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12140.21877066743074214No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT12090.2178696350278149No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA11830.21318426653259304No Hit
TCCTACACCAACGGCAAACTGACTATCACCC11550.20813848507620034No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11380.20507497490624757No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11020.1985875416051712No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10990.19804692216341485No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10820.1949834119934621No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10640.19173969534292393No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG10610.19119907590116755No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA10550.19011783701765483No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9880.17804400281842936No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9750.17570131857081844No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9630.17353884080379298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9570.17245760192028026No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9330.16813264638622932No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9160.1650691362162766No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC8720.1571400510705166No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8530.15371612793939296No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8520.1535359214588075No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8290.14939117240534205No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8220.14812972704124386No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8010.1443453909489493No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7940.14308394558485113No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7520.13551527340026204No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7430.13389341507499292No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7360.13263196971089475No Hit
TGCCAGCACCCGGAACCGCTGGCTTGCCCAG7330.13209135026913837No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG7270.13101011138562565No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7100.12794660121567292No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7080.127586188254502No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA6560.11821545126405836No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6490.11695400589996018No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6180.11136760500181109No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG6050.10902492075420016No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5960.10740306242893106No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5890.1061416170648329No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5880.10596141058424743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)