FastQCFastQC Report
Wed 31 May 2023
EGAF00007962521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539581
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT73811.3679132512078818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42080.7798643762474957No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33370.6184428287875221No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23830.44163897542722963No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG23790.4408976594802263No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23080.4277393014209174No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21050.3901175171104987No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19290.3574996154423525No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18410.34119066460827935No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18030.3341481631117478No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16570.30709013104612654No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15960.2957850628543258No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15590.28892789034454514No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15590.28892789034454514No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15100.2798467699937544No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13870.2570513046234022No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11960.22165346815399356No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11490.21294300577670452No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA11240.20830978110793372No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11220.20793912313443208No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10800.2001553056908972No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10600.1964487259558806No Hit
TCCTACACCAACGGCAAACTGACTATCACCC10600.1964487259558806No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10580.19607806798237892No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10060.18644096067133573No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9970.18477299979057826No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9570.177359840320545No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA9530.1766185243735417No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG9350.17328260261202674No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8980.166425430102246No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8940.16568411415524267No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8870.16438681124798687No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8620.1597535865792161No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8400.1556763488706978No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8000.1482631894006646No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7940.1471512154801596No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7820.14492726763914965No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7710.1428886487848905No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7700.1427033197981397No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC7620.14122068790413303No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7580.1404793719571297No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7370.13658746323536225No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7250.1343635153943523No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG7020.13010094869908317No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6890.12769167187132238No Hit
TGCCAGCACCCGGAACCGCTGGCTTGCCCAG6420.11898120949403333No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6180.1145333138120134No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG5950.11027074711674428No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5910.10952943116974098No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA5870.10878811522273765No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5870.10878811522273765No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5830.10804679927573432No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5830.10804679927573432No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5720.1060081804214752No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5430.1006336398057011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)