FastQCFastQC Report
Sun 21 May 2023
EGAF00007962539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514903
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA28340.5503949287535711No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17650.3427830096154033No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA17530.3404524735726923No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA15170.2946185980660435No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14860.28859804662237354No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14680.2851022425583071No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13810.2682058562486527No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12880.25014420191764275No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT12810.24878472255939468No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11950.23208254758663285No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11180.21712827464590417No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10720.20819455314884552No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10470.20333926972653105No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10450.20295084705274588No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC9490.1843065587110582No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9250.17964548662563629No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG9030.17537283721399952No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8450.1641085796742299No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8330.16177804363151893No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG7880.15303853347135285No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7800.15148484277621221No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7180.13944373988887226No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6860.13322897710830972No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA6830.13264634309763199No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6340.12312998758989557No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6290.12215893090543267No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6270.12177050823164752No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG6040.1173036474831182No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6000.11652680213554786No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5790.11244836406080368No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5780.1122541527239111No Hit
ATGTCTTCTCGCCGCCGTAGCTCTTCCCGTC5670.11011782801809272No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5640.10953519400741499No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5590.10856413732295209No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5400.10487412192199308No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5280.10254358587928213No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC5260.10215516320549696No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5240.10176674053171181No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5210.10118410652103407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)