FastQCFastQC Report
Sun 21 May 2023
EGAF00007962544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498840
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT13840.27744366931280573No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG13450.2696255312324593No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13390.2684227407585599No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG13040.26140646299414644No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13040.26140646299414644No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12680.25418972015074975No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG12370.24797530270226925No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG10480.21008740277443672No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10020.2008660091412076No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9160.18362601234864886No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9110.18262368695373266No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA8770.17580787426830247No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA8670.17380322347847005No Hit
GTCGAAAGCGACGGTTTTCATGCCGGTGGTGGATTTCACCGGGC8440.1691925266618555No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG8060.16157485366049235No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG7880.157966482238794No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7480.14994787907946436No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC7000.1403255552882688No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6810.1365167187875872No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT6490.13010183626012348No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6320.12669392991740838No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6240.12509020928554246No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT6140.12308555849571004No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT6030.12088044262689439No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5970.11967765215299495No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG5950.11927672199502848No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5790.1160692807312966No Hit
CATACGGTCCAGGTCTTCGGTCGCGGAGCGAGCACGGGTGCTGG5780.11586881565231336No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT5750.11526742041536366No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA5740.1150669553363804No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC5610.11246090930959826No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5600.11226044423061501No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG5580.11185951407264853No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5460.10945393312484966No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5420.1086520728089167No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5240.10504370138721836No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG5200.10424184107128537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)