FastQCFastQC Report
Wed 8 May 2024
EGAF00007962586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521633
Total Bases22.9 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20920.4010482465641552No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT19760.3788103896801008No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG15000.2875584941903599No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13460.2580358221201496No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG12350.23675649355006295No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11360.21777763293349922No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9720.1863379042353532No Hit
ACGGCTGAAGCACAGCTGGCAGCCGATGTGGTGCATTTTCAGAC9650.1849959645957982No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9520.18250379097948174No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9470.18154526266551388No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8950.17157656820024808No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG8940.1713848625374545No Hit
GGCTGCCGGTTTAGCCGCCGGCTTGGCCGCTGCGGACTTAGCAA8910.17080974554907377No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8660.16601710397923444No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8560.1641000473512987No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG7850.150488945292955No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT7780.1491470056534No Hit
ACGACGAGAACGGCGAGAGCCTGCGGACTCACCCTGACCGGACT7720.14799677167663855No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7710.147805066013845No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG7390.14167048480445063No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7380.14147877914165705No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7020.13457737528108843No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6960.13342714130432698No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6770.1297847337112491No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG6320.12115797888553831No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6180.11847409960642828No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5960.11425657502496966No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5860.11233951839703393No Hit
GAACGGCAGGCTAGAACCACGGCGGCCGCGACGACCTTCTTTTT5760.11042246176909819No Hit
GTTTACCGGCAGTTCGCTCACGCCGTGTTTAGTGGAGTCACGCA5460.10467129188529099No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5360.10275423525735526No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5260.10083717862941954No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5230.1002620616410388No Hit
AGCACCTGCAACGGTACCGGTGCCGTTGTCGCCCTGCGCGATGC5230.1002620616410388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)