FastQCFastQC Report
Wed 8 May 2024
EGAF00007962587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521633
Total Bases16.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20740.397597544633871No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG20310.3893542011337473No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18720.3588730007495692No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT14820.2841077922600756No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14750.28276585262052056No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13730.2632118750155761No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13000.2492173616316452No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC12170.23330579161977863No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11410.2187361612474671No Hit
GCTAGCCGTGAACATTTCGCGATGCAGGGTC10250.1964983043634126No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10170.194964659061064No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9450.18116185133992674No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9190.17617750410729383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9160.1756023871189131No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA8790.16850927759555087No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC8710.1669756322932023No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8700.16678392663040875No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8230.1577737604791108No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7690.14742165468825782No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC7680.14722994902546427No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG7500.14377924709517995No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7360.14109536781606993No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7190.13783637154857917No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7030.13476908094388199No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6830.13093496768801055No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6740.12920961672286838No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6510.12480038647861619No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6450.12365015250185477No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5920.11348975237379538No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5540.10620493718763958No Hit
GCAGTCAACGAACTGACCTTCCCGGGTTCTG5530.106013231524846No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5480.10505470321087815No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5480.10505470321087815No Hit
ATGACTCAGCGTAACGAGAAAAGCATGGGTA5470.10486299754808458No Hit
TGGCAGGGTCTGAGCCTGGATGATGGTCAGG5320.10198741260618097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)