FastQCFastQC Report
Wed 8 May 2024
EGAF00007962588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507131
Total Bases22.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19310.3807694658776529No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT18640.3675578893816391No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG13810.2723162259850019No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13090.2581187109445094No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG12410.2447099467395998No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10770.21237116248070026No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10660.21020209768284723No Hit
ACGGCTGAAGCACAGCTGGCAGCCGATGTGGTGCATTTTCAGAC9970.19659614576904194No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9430.18594800948867257No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9200.18141269218407077No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8940.17628581175278182No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG8630.17017299277701423No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8160.16090517045891498No Hit
GGCTGCCGGTTTAGCCGCCGGCTTGGCCGCTGCGGACTTAGCAA8060.15893329336995765No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7890.15558110231873026No Hit
ACGACGAGAACGGCGAGAGCCTGCGGACTCACCCTGACCGGACT7860.15498953919204309No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7710.15203172355860714No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG7690.1516373481408157No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT7140.1407920241515506No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7100.14000327331596768No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG6850.13507358059357444No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG6590.12994670016228546No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6460.12738325994664101No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6310.12442544431320508No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6290.1240310688954136No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6240.12304513035093496No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6180.12186200409756058No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5980.11791824991964601No Hit
GTTTACCGGCAGTTCGCTCACGCCGTGTTTAGTGGAGTCACGCA5450.10746730134817237No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5180.1021432332079877No Hit
GAACGGCAGGCTAGAACCACGGCGGCCGCGACGACCTTCTTTTT5130.10115729466350903No Hit
AGCACCTGCAACGGTACCGGTGCCGTTGTCGCCCTGCGCGATGC5130.10115729466350903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)