FastQCFastQC Report
Wed 8 May 2024
EGAF00007962598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527203
Total Bases23.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG60721.151738514386299No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC35380.6710887457013712No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT34270.6500342372862067No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC26600.5045494809399795No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG19020.3607718468976846No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18670.35413303793794804No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16880.32018027211529526No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16400.3110756198276565No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC15690.29760832165219087No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG15450.2930559955083715No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12770.24222168690238863No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG12360.23444479640669724No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT12110.22970279000688537No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG11660.22116717848722406No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11490.21794261413535204No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG10700.20295787391194664No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG10340.1961293846962176No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG10000.18968025599247348No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9540.1809549642168197No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9390.1781097603769326No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC9140.17336775397712076No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG8720.1654011832254369No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8440.16009013605764763No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8280.15705525196176806No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG8260.1566758914497831No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC8170.15496876914585084No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8010.15193388504997127No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7410.14055306969042283No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT7190.13638010405858844No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG7150.13562138303461854No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG6910.1310690568907992No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6690.12689609125896478No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC6590.12499928869904003No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6520.12367152690709272No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6360.12063664281121314No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT6350.12044696255522067No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6150.1166533574353712No Hit
AGTTGGCAGCTGTTCATCACCGCTGTCGGAACGGATGTAGGTTT6120.11608431666739379No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC5850.11096294975559699No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5790.10982486821964216No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG5690.10792806565971741No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5510.10451382105185289No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5470.10375510002788299No Hit
ACGACCTTCAACGGATTTTTCAACTACTTTACCGTCTTTTTTGG5320.10090989618799588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)