FastQCFastQC Report
Wed 8 May 2024
EGAF00007962599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527203
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA77651.4728671877815565No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG58551.1105778988359323No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA39570.7505647729622176No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35760.6782965954290853No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19740.3744288253291427No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA18850.35754728254581253No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG18060.3425625423224071No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG16520.3133517828995662No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16260.3084200962437619No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG13510.2562580258458317No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12800.24279072767036608No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12760.24203200664639615No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12300.2333067148707424No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12280.23292735435875744No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12250.23235831359078005No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA11860.22496078360707356No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11060.20978636312767568No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG10480.19878490828011222No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10460.19840554776812727No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9770.1853176101046466No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC9490.18000656293685732No Hit
GAACCGATCTACGCTACCATCGACGACCTGG9360.17754071960895518No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9280.1760232775610154No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA9260.17564391704903043No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9160.17374711448910574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9100.17260903295315086No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8670.16445278194547452No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC7990.15155452453798632No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7520.14263955250634006No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7440.14112211045840029No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7300.13846658687450566No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC7210.1367594645705734No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7130.13524202252263362No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG7020.1331555397067164No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA7010.1329658594507239No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6730.12765481228293465No Hit
GGCAAACGTCCGTCTATTGGTAACCTGGAAC6430.12196440460316045No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6350.12044696255522067No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6300.1194985612752583No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5990.11361847333949161No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5980.11342879308349914No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5930.11248039180353678No Hit
AAACGTGTCTCCGGCTTCGACGTGGTACTGA5740.10887646693967978No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC5640.10697966437975505No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5410.10261701849192816No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5410.10261701849192816No Hit
CTGGACGCACTGGGCAACGATCGCATCGCGT5410.10261701849192816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)