FastQCFastQC Report
Wed 8 May 2024
EGAF00007962603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525065
Total Bases16.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA77711.480007237199204No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG58331.1109100777998915No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA40050.7627627055697866No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34800.6627750849894776No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA19480.3710016855056041No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19370.3689067067886833No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG18150.3456714882919258No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG17290.329292563777818No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16380.3119613762105644No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG13640.2597773608981745No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13350.2542542351899289No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12880.24530296249035835No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12570.23939893156085437No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12490.23787531067582107No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11630.2214963861617133No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11570.22035367049793833No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA11110.21159285040899697No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10810.2058792720901222No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10430.19864207288621408No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG10330.19673754677992247No Hit
GAACCGATCTACGCTACCATCGACGACCTGG9780.18626265319531868No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9720.1851199375315437No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC9690.18454857969965624No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC9300.177120927885119No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9210.17540685438945652No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA9070.17274051784064828No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8410.16017064553912375No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC7890.1502671097864074No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC7570.14417262624627428No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7460.1420776475293535No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA7200.13712587965299536No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7180.13674497443173703No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7150.13617361659984956No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC7030.13388818527229962No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6950.13236456438726632No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6780.1291268700065706No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6630.1262700808471332No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6270.11941378686448345No Hit
GGCAAACGTCCGTCTATTGGTAACCTGGAAC6170.11750926075819183No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6150.11712835553693353No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC5930.112938398103092No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5800.11046251416491291No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5700.1085579880586213No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5680.108177082837363No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5350.10189214668660071No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5310.10113033624408406No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5280.10055897841219658No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA5260.10017807319093826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)