FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007962604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511489
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG57771.1294475541018478No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC33940.6635528818801578No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT33650.6578831607326844No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC25430.4971758923456809No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18400.3597340314258958No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG17490.3419428374803759No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16390.32043699864513214No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16050.31378973936878407No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC15300.29912666743566335No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG15210.29736709880368883No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12400.24242945596092977No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11840.23148102891753292No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG11460.2240517391380851No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG11070.2164269417328623No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10480.20489199181214063No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG9950.19453008764606863No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG9860.19277051901409414No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9570.1871007978666208No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9320.1822131072222472No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG9230.18045353859027272No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC9020.1763478784489989No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG8840.17282874118504993No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG8630.1687230810437761No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8610.16833206579222623No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG7880.15406000911065534No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7820.1528869633560057No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7640.14936782609205673No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC7500.14663071933120753No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG6980.13646432279091045No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6920.13529127703626082No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT6880.13450924653316101No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG6520.12747097200526308No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC6280.12277878898666443No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6050.11828211359384073No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC5940.11613152971031634No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5900.11534949920721659No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG5860.1145674687041168No Hit
AGTTGGCAGCTGTTCATCACCGCTGTCGGAACGGATGTAGGTTT5810.11358993057524208No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5790.1131989153236922No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5710.11163485431749265No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA5610.10967977805974322No Hit
TTTAGCTTCACGACCACGAGAACGAGAGCGGGCGCGACGGGTTG5590.10928876280819333No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5570.10889774755664344No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC5500.10752919417621884No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5340.10440107216381975No Hit
ACGACCTTCAACGGATTTTTCAACTACTTTACCGTCTTTTTTGG5210.10185947302874548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)