FastQCFastQC Report
Wed 8 May 2024
EGAF00007962654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486065
Total Bases21.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA46370.9539876353985578No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA44260.9105778033802063No Hit
GGTGCGTTTACGTACCGGCTGACGGCGCTGCGGCAGCGGGGTCG24120.4962299280960365No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT19950.4104389330645078No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15380.3164185859915855No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15120.3110695071646796No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC14480.2979025438984498No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13910.28617571723946383No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12160.25017230205836666No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11210.2306275909600568No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10330.21252301646899077No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG9980.20532233343277134No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9820.20203059261621387No Hit
CTGCAGCTCGCGAGCGCTGATGTAACCATCGCCATCTTCATCGA9410.1935955067737854No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9320.19174390256447182No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7990.16438130702683798No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7660.15759209159268822No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7390.1520372789647475No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7010.14421939452542357No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6920.14236779031611No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6720.13825311429541315No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6670.13722444529023894No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6550.13475563967782087No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG6380.13125816506022858No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6300.12961229465194984No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5740.11809120179399875No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5630.11582812998261549No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5600.11521092857951097No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5300.10903891454846575No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5240.1078045117422567No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5220.10739304414018702No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5190.1067758427370825No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5140.10574717373190828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)