FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007962655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486065
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT101262.0832604692788004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42730.8791005318218756No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33010.6791272772160103No Hit
CACCAGCCGATGCGTGAACGTGCACAGTCTA24700.5081624885560574No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21230.4367728595969675No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16460.33863783650334833No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16410.3376091674981741No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14360.2954337382860317No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13610.28000370320841866No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11630.2392684106035201No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10010.20593953483587585No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC9640.19832738419758672No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG9210.18948083075308858No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8260.1699361196547787No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7360.151420077561643No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7140.1468939339388765No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA7050.14504232972956294No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6810.14010471850472672No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6780.13948751710162222No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6770.13928178330058738No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6470.13310976926954216No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6260.12878935944781048No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6110.12570335243228786No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6060.12467468342711366No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.1168567989877897No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG5610.1154166623805458No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5450.11212492156398834No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5400.11109625255881414No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5280.10862744694639606No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5090.10471850472673408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)