FastQCFastQC Report
Wed 8 May 2024
EGAF00007962657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503893
Total Bases15.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT100611.9966540515545959No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA35150.6975687298692381No Hit
CACCAGCCGATGCGTGAACGTGCACAGTCTA26090.5177686532656735No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21680.43025007293215023No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18340.363966159482271No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17450.34630367955101576No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC16020.31792463876259447No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14350.28478268203765483No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12830.25461754777303913No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10520.2087744818840508No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG10290.20421002077822079No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC9130.1811892604183825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8860.1758309799897994No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8420.1670989674395159No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7860.1559854969209733No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7710.1530086744606494No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7660.1520164003072081No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7420.1472534843706898No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6920.13733074283627675No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6720.13336164622251154No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6660.13217091723838195No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6640.13177400757700541No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6470.128400275455305No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6330.12562190782566934No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6280.12462963367222803No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6170.12244663053465717No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6060.12026362739708629No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5830.11569916629125628No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5730.11371461798437366No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5310.1053795150954667No Hit
GACGACAACACCAAGCCATGGAAACAGCTGT5250.10418878611133714No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5040.10002123466688366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)