FastQCFastQC Report
Wed 8 May 2024
EGAF00007962658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491077
Total Bases21.6 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA46640.949749224663342No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA44500.9061715372538319No Hit
GGTGCGTTTACGTACCGGCTGACGGCGCTGCGGCAGCGGGGTCG23810.48485268094412887No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT20120.4097117152707213No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC16020.3262217534113795No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15360.3127819058925586No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14320.29160396434775No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC13850.28203316384192295No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12620.2569861752841204No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12150.24741537477829342No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10780.2195175094740743No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG10370.21116851328814015No Hit
CTGCAGCTCGCGAGCGCTGATGTAACCATCGCCATCTTCATCGA10180.20729946627514625No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10000.20363405331546786No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9960.20281951710220597No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7730.15740912321285666No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7590.1545582464664401No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7470.15211463782665446No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7390.15048556540013075No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7240.1474310546003987No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6880.14010022868104186No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6720.1368420838279944No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6360.12951125790863754No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5900.12014409145612603No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5850.11912592118954869No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5600.114035069856662No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5510.11220236337682278No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5490.11179509527019184No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5420.11036965689698355No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5380.1095551206837217No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5150.10487153745746594No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5030.10242792881768033No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5010.10202066071104939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)