FastQCFastQC Report
Wed 8 May 2024
EGAF00007962659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491077
Total Bases15.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT107872.1966005331139513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41850.8522085131252329No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33850.6893012704728587No Hit
CACCAGCCGATGCGTGAACGTGCACAGTCTA24820.5054197203289912No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21170.4310932908688454No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16830.3427161117299324No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16470.33538528581057553No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14940.30422927565330893No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13990.2848840405883395No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12470.25393166448438836No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10400.21177941544808657No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG10220.20811400248840814No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC9840.20037590846242034No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7940.16168543833248147No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7310.148856492973607No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7280.14824559081366057No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7070.14396927569403575No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7020.14295110542745842No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6900.1405074967876728No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6640.13521301140147066No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6510.13256576870836956No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6380.1299185260152685No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6280.1278821854821138No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6170.12564221089564367No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.11566414228318574No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5660.1152568741765548No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5540.11281326553676918No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5510.11220236337682278No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5490.11179509527019184No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5450.11098055905692998No Hit
GACGACAACACCAAGCCATGGAAACAGCTGT5220.1062969758306742No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5170.10527880556409687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)