FastQCFastQC Report
Wed 8 May 2024
EGAF00007962670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540744
Total Bases23.7 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG27220.5033805275694229No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23740.4390247510836921No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA23410.43292204814107965No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22370.4136892873522406No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA21260.3931620138179989No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19430.3593197520453301No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17810.3293610285088692No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG16900.31253236281863506No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16300.3014365392866125No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG14800.27369698045655616No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14570.2694435814359475No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG14300.2644504608465374No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12810.2368958324086814No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12140.2245054961312562No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11640.21525897652123743No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11640.21525897652123743No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA11240.20786176083322236No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10660.19713579808560058No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9700.17938248043436453No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9140.16902637847114346No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8510.1573757637625198No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7370.13629369905167693No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7040.13019099610906454No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG6850.12667731865725743No Hit
GAAACCGAACGGAATGCGTTTGCCTTCTACGTTCTGCTGGCCAA6780.12538280591185477No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG6650.1229787108132499No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6630.12260885002884914No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG6540.12094447649904576No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6330.11706093826283787No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6110.1129924696344296No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6060.11206781767342772No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG6030.11151302649682658No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5950.11003358335922359No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5870.10855414022162059No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5800.10725962747621795No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5470.10115692453360554No Hit
AGCGGTGTGGGTGATGAAGATAACGTCCGCACCGTCCTTTACGG5450.10078706374920479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)