FastQCFastQC Report
Wed 8 May 2024
EGAF00007962981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007962981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521858
Total Bases16.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20330.38956957639817724No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17120.32805859065109666No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12240.23454656247484948No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11990.22975598726090238No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA11840.22688164213253412No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11520.22074970585868187No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11110.21289316250780863No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10630.2036952580970302No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8900.17054447761651637No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8160.156364374983233No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7620.14601673252110728No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7570.14505861747831786No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7520.14410050243552844No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6850.13126176086215025No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6680.12800416971666623No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6560.12570469361397163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6050.11593192017751955No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5710.10941673788655151No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5420.1038596706383729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)