FastQCFastQC Report
Wed 31 May 2023
EGAF00007963046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470522
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA24370.5179353994074666No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22330.4745792970360578No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16040.34089798139088073No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14620.3107187336617629No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13030.2769264774016943No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12310.26162432362355004No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10600.22528170840045736No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9940.21125473410382511No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9120.19382728118982748No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8900.18915162309095004No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8590.1825631957698046No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7590.1613102044112709No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7420.15769719588032013No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7400.1572721360531495No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7100.15089623864558938No Hit
CGCCGGAACTTCTTTAGAGTGAGCTGGACCGGATGCTTTAGTGT7030.149408529250492No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6990.14855840959615066No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6920.14707070020105328No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6620.14069480279349317No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6580.13984468313915183No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6560.13941962331198116No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6300.13389384555876238No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6260.13304372590442104No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6240.13261866607725037No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6230.13240613616366503No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6040.12836806780554363No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT5870.1247550592745929No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5830.12390493962025155No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5660.12029193108930082No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5600.1190167516077888No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5590.11880422169420347No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5440.11561627299042339No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5320.11306591402739936No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5270.11200326445947267No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5170.1098779653236193No Hit
ATTGGCTGCTTGGGTAGCCAGTTCGTTCATGCGCTGCAGAATGC4970.10562736705191256No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4880.10371459782964454No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG4830.10265194826171785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)