FastQCFastQC Report
Wed 31 May 2023
EGAF00007963050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474875
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25400.5348775993682547No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22800.48012634903922086No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16800.35377730981837324No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15860.3339826270071071No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13330.2807054488023164No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13320.28049486707028165No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9750.20531718873387736No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9590.2019478810213214No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9560.20131613582521718No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8980.1891023953672019No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8800.18531192419057646No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7840.16509607791524086No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7610.1602526980784417No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7380.15540931824164253No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7290.15351408265332983No Hit
CGCCGGAACTTCTTTAGAGTGAGCTGGACCGGATGCTTTAGTGT7240.1524611739931561No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7180.15119768360094762No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6960.14656488549618318No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6760.14235325085548828No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6560.13814161621479337No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6090.1282442748091603No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6070.1278231113450908No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6050.12740194788102133No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6030.12698078441695182No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5960.12550671229270863No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5900.12424322190050013No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5860.12340089497236116No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5660.11918926033166621No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT5600.11792576993945776No Hit
ATTGGCTGCTTGGGTAGCCAGTTCGTTCATGCGCTGCAGAATGC5600.11792576993945776No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5600.11792576993945776No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5550.11687286127928403No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5430.11434588049486706No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5430.11434588049486706No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5280.11118715451434588No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5280.11118715451434588No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5210.10971308239010266No Hit
GCTAGAGGCCTGAGACAGGCCGCGGATCTGCTTACGCATTTTTT5060.10655435640958147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)