FastQCFastQC Report
Wed 8 May 2024
EGAF00007963052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455490
Total Bases20 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA24420.5361259303168017No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA22760.4996816615073877No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15740.3455619223254078No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14920.3275593317087093No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12210.26806296515840083No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12200.26784342137039235No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9290.20395617905991348No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9160.20110210981580276No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8900.1953939713275813No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8320.18266043162308723No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8090.1776109244988913No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7190.15785198357812466No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7180.15763243979011612No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7070.15521745812202245No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7000.1536806516059628No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6830.149948407209818No Hit
CGCCGGAACTTCTTTAGAGTGAGCTGGACCGGATGCTTTAGTGT6770.1486311444817669No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6710.14731388175371576No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6620.1453379876616391No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6250.13721486750532394No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6180.1356780609892643No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6150.13501942962523875No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6020.13216536038112803No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT5900.12953083492502582No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5830.12799402840896618No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5750.12623767810489803No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5710.12535950295286394No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5610.12316406507277876No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5600.12294452128477024No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5500.12074908340468506No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5480.12030999582866803No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5400.11855364552459988No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5370.11789501416057432No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4800.10538101824408878No Hit
ATTGGCTGCTTGGGTAGCCAGTTCGTTCATGCGCTGCAGAATGC4790.10516147445608028No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG4770.10472238688006323No Hit
GCTAGAGGCCTGAGACAGGCCGCGGATCTGCTTACGCATTTTTT4750.1042832993040462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)