FastQCFastQC Report
Wed 8 May 2024
EGAF00007963053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455490
Total Bases14.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58691.288502491821994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54241.1908055061582032No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA26360.5787174251904542No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17550.38529934795494963No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17290.37959120946672814No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16290.3576368306658763No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC13050.28650464335111636No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12600.2766251728907331No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11090.2434740609014468No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10390.2281059957408505No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9340.20505389799995608No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8820.19363762102351315No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8570.1881490263233002No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7780.17080506707062723No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7520.16509692858240577No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG7440.16334057827833762No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7200.15807152736613317No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7140.15675426463808206No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7000.1536806516059628No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG7000.1536806516059628No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6560.144020724933588No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6420.14094711190146875No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6350.1394103053854091No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5890.12931129113701728No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5840.12821357219697468No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5830.12799402840896618No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5720.12557904674087247No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5500.12074908340468506No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5390.11833410173659137No Hit
GCGGCTGGTCTGGCAATTTCTCAGCGCATGA5240.11504094491646358No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5020.11021098158027619No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5000.10977189400425914No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC4930.10823508748819952No Hit
ATCAACCGTGCAGCGGACGATGCGGCTGGTC4910.10779599991218249No Hit
ATGGTGGTTCAGCACAACCTGACTGCGATGA4730.10384421172802914No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4640.10186831763595249No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4590.10077059869590992No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4580.10055105490790138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)